The figure of the Virgin Mary standing, with the prayer book in hand, stands out, almost like a cultured lady of the Renaissance courts, inside a basilica building with a perspective flight of columns, in the act of receiving the announcement from the Angel, of her divine motherhood. The two canvases originally constituted the doors of the organ, commissioned by Julius II and made under Leo X, which was located above the entrance door to the Sacristy of St. Mark, frescoed by Melozzo da Forlì. At the end of the eighteenth century, when their practical function was over, the canvases were hung in the old bishop’s major sacristy, now the Holy House Library, from which they were removed in 1888, at the request of the district inspector, Pietro Gianuizzi, to be exhibited in the rooms of the Museum. For their testing, in 1515, Luca Signorelli was called from Cortona.